anti icos mab  (Bio X Cell)

Journal: Frontiers in Immunology

Article Title: CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis

doi: 10.3389/fimmu.2022.1056397

Figure Lengend Snippet: Pulmonary melanoma metastasis inhibits T cell co-stimulation. WT mice were given B16-F10 (B16) melanoma cells or control vehicle (PBS) and evaluated 2 weeks later. (A–C) Real-time RT-PCR (RT-PCR) was used to quantitate the levels of mRNA encoding ICOS, ICOSL and CD28 in the lungs from mice treated intravenously with PBS (B16 -) or B16 cells (B16 +). Each dot represents an evaluation in an individual animal. (D) Western blot and densitometry evaluations of ICOS, ICOSL and CD28 accumulation in lungs from mice treated with PBS (B16 -) or B16 cells (B16 +). (E, F) FACS evaluations quantitating the accumulation of ICOS and CD28 in cell populations from lungs of mice treated with B16 cells (B16+) or vehicle control (B16 -). These evaluations used specific markers of airway myeloid cells. The values in panel A represent the mean±SEM of the noted evaluations. Panels (D–F) are representative of a minimum of 2 similar evaluations. * P < 0.05, ** P < 0.01 ( t- Test).

Article Snippet: Anti-mouse ICOS (7E.17G9, Bio X Cell) and anti-mouse ICOSL (HK5.3, Bio X Cell), anti-mouse CD28 (37.51, Bio X Cell), anti-human-CTLA-4 (922101, R&D), anti-mouse-CTLA-4 (a generous gift from Prof. Lieping Chen, Medical Oncology, Yale School of Medicine), anti-mouse B7-1 (F-7, Santa Cruz Biotechnology) and anti-mouse B7-2 (BU63, Santa Cruz Biotechnology) and b-actin (C4, Santa Cruz Biotechnology) were used as primary antibodies and immunoreactive bands were captured and analyzed using a ChemiDoc (Bio-Rad) imaging system.

Techniques: Control, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Frontiers in Immunology

Article Title: CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis

doi: 10.3389/fimmu.2022.1056397

Figure Lengend Snippet: CHI3L1 plays a critical role in B16 cell regulation of T cell co-stimulators and the induction of CTLA-4 and its ligands. WT ( Chil1 +/+ ) and Chi3l1 null ( Chil1 -/- ) mice were used to evaluate the expression and accumulation of co-stimulators (ICOS, ICOS L CD28) and CTLA-4, B7-1 and B7-2 in the lung. (A) RT-PCR was used to quantitate the levels of mRNA encoding ICOS, ICOS L and CD28 in the lungs from WT mice and Chil1 -/- mice randomized to receive B16 cells (B16+) or control vehicle (B16-). (B) RT-PCR was used to quantitate the levels of mRNA encoding CTLA-4, B7-1and B7-2 in the lungs from WT mice and Chil1 -/- mice randomized to receive B16 cells (B16+) or control vehicle (B16-). (C, D) RT-PCR was used to quantitate the levels of mRNA encoding ICOS, ICOS L, CD28 CTLA-4, B7-1 and B7-2 in the lungs from WT mice randomized to receive B16 cells (B16+) or vehicle control (B16-) and randomized to receive the FRG antibody (FRG+) or an isotype antibody control (FRG-). (E) RT-PCR was used to quantitate the levels of mRNA encoding CTLA-4, B7-1 and B7-2 in the lungs from WT mice and Chi3l1 transgenic ( Chi3l1 Tg) mice randomized to receive B16 cells (B16+) or vehicle control (B16-). In all panels each dot represents the evaluation in an individual animal. The values in these panels represent the mean±SEM of the noted evaluations represented by the individual dots. * P <0.05. ** P <0,01, *** P <0.005 (ANOVA with multiple comparisons).

Article Snippet: Anti-mouse ICOS (7E.17G9, Bio X Cell) and anti-mouse ICOSL (HK5.3, Bio X Cell), anti-mouse CD28 (37.51, Bio X Cell), anti-human-CTLA-4 (922101, R&D), anti-mouse-CTLA-4 (a generous gift from Prof. Lieping Chen, Medical Oncology, Yale School of Medicine), anti-mouse B7-1 (F-7, Santa Cruz Biotechnology) and anti-mouse B7-2 (BU63, Santa Cruz Biotechnology) and b-actin (C4, Santa Cruz Biotechnology) were used as primary antibodies and immunoreactive bands were captured and analyzed using a ChemiDoc (Bio-Rad) imaging system.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Transgenic Assay

Journal: Frontiers in Immunology

Article Title: CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis

doi: 10.3389/fimmu.2022.1056397

Figure Lengend Snippet: Chi3l1 inhibits co-stimulation and stimulates pulmonary CTLA-4 in the normal lung. WT and Chi3l1 Tg (+) mice were used to evaluate the effects of transgenic Chi3l1 on co-stimulation and the CTLA-4 axis. (A) RT-PCR was used to quantitate the levels of mRNA encoding ICOS, ICOS L and CD28 in the lungs from WT mice and Chi3l1 Tg+ mice. (B) RT-PCR was used to quantitate the levels of mRNA encoding CTLA-4, B7-1and B7-2 in the lungs from WT mice and Chi3l1 Tg+ mice. (C) Western blot and densitometry evaluations of ICOS, ICOSL, CD28 B7-1 and B7-2 accumulation in lungs from WT and Chi3l1 Tg+ mice. (D) FACS evaluations quantitating the accumulation of CTLA-4 in CD3+ cells from WT mice and Chi3l1 Tg+ mice. (E) Cumulative FACS evaluations quantitating the accumulation of CTLA-4 in CD3+ cells from WT and Chi3l1 Tg+ mice. The plotted values in panels (A, B, E) represent the mean ± SEM of the noted evaluations represented by the individual dots. Panel C is representative of a minimum of 2 similar evaluations. * P < 0.05, ** P < 0.01 ( t -Test for panels (A, B) , ANOVA with multiple comparisons for panels E ).

Article Snippet: Anti-mouse ICOS (7E.17G9, Bio X Cell) and anti-mouse ICOSL (HK5.3, Bio X Cell), anti-mouse CD28 (37.51, Bio X Cell), anti-human-CTLA-4 (922101, R&D), anti-mouse-CTLA-4 (a generous gift from Prof. Lieping Chen, Medical Oncology, Yale School of Medicine), anti-mouse B7-1 (F-7, Santa Cruz Biotechnology) and anti-mouse B7-2 (BU63, Santa Cruz Biotechnology) and b-actin (C4, Santa Cruz Biotechnology) were used as primary antibodies and immunoreactive bands were captured and analyzed using a ChemiDoc (Bio-Rad) imaging system.

Techniques: Transgenic Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Frontiers in Immunology

Article Title: CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis

doi: 10.3389/fimmu.2022.1056397

Figure Lengend Snippet: Rig-like helicase (RLH) activation augments co-stimulation and inhibits CTLA-4, B7-1 and B7-2. WT mice were used to evaluate the effects of Rig-like helicase activation with Poly (I:C) on co-stimulation and the CTLA-4 axis. (A) RT-PCR was used to quantitate the levels of mRNA encoding ICOS, ICOS L and CD28 in the lungs from WT mice that received B16 cells (B16+) or vehicle control (B16-) and were treated with Poly (I:C) (+) or its vehicle control (Poly(I:C)-). (B) RT-PCR was used to quantitate the levels of mRNA encoding CTLA-4, B7-1 and B7-2 in the lungs from WT mice that received B16 cells (B16+) or vehicle control (B16-) and were treated with Poly (I:C) (+) or its vehicle control (Poly(I:C)-). (C) FACS evaluations comparing the expression of CTLA-4 on CD3+ cells from WT mice that received B16 cells (B16+) or vehicle control (B16-) and were treated with Poly (I:C) or its vehicle control. (D) FACS evaluations comparing the expression of CD28 on CD11b+ cells from WT mice that received B16 cells (B16+) or vehicle control (B16-) and were treated with Poly (I:C) or its vehicle control. The plotted values in panels (A, B) represent the mean ± SEM of the noted evaluations represented by the individual dots. Panels C and D are representative of a minimum of 2 similar evaluations. * P < 0.05, ** P < 0.01, *** P < 0.001 (ANOVA with multiple comparisons).

Article Snippet: Anti-mouse ICOS (7E.17G9, Bio X Cell) and anti-mouse ICOSL (HK5.3, Bio X Cell), anti-mouse CD28 (37.51, Bio X Cell), anti-human-CTLA-4 (922101, R&D), anti-mouse-CTLA-4 (a generous gift from Prof. Lieping Chen, Medical Oncology, Yale School of Medicine), anti-mouse B7-1 (F-7, Santa Cruz Biotechnology) and anti-mouse B7-2 (BU63, Santa Cruz Biotechnology) and b-actin (C4, Santa Cruz Biotechnology) were used as primary antibodies and immunoreactive bands were captured and analyzed using a ChemiDoc (Bio-Rad) imaging system.

Techniques: Activation Assay, Reverse Transcription Polymerase Chain Reaction, Control, Expressing

Journal: Frontiers in Immunology

Article Title: CHI3L1 enhances melanoma lung metastasis via regulation of T cell co-stimulators and CTLA-4/B7 axis

doi: 10.3389/fimmu.2022.1056397

Figure Lengend Snippet: Effects of transgenic Chi3l1 on Rig-like helicase (RLH) inhibition of co-stimulation and induction of CTLA-4, B7-1 and B7-2. WT mice and Chi3l1 Tg+ mice were given B16-F10 (B16+) melanoma cells or PBS control (B16-) and treated with Poly(I:C) (Poly (I:C+) or its vehicle control (Poly(I:C-) and evaluated 2 weeks later. (A-F) RT-PCR was used to quantitate the levels of mRNA encoding ICOS, ICOSL, CD28, CTLA-4, B7-1 and B7-2 in lungs from WT mice and Chi3l1 Tg+ mice treated with Poly(I:C) (Poly (I:C+) or its vehicle control (Poly (I:C-) as noted. The plotted values in panels (A–F) represent the mean±SEM of the evaluations represented by the individual dots. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P <0.0001. (ANOVA with multiple comparisons). ns, not significant.

Article Snippet: Anti-mouse ICOS (7E.17G9, Bio X Cell) and anti-mouse ICOSL (HK5.3, Bio X Cell), anti-mouse CD28 (37.51, Bio X Cell), anti-human-CTLA-4 (922101, R&D), anti-mouse-CTLA-4 (a generous gift from Prof. Lieping Chen, Medical Oncology, Yale School of Medicine), anti-mouse B7-1 (F-7, Santa Cruz Biotechnology) and anti-mouse B7-2 (BU63, Santa Cruz Biotechnology) and b-actin (C4, Santa Cruz Biotechnology) were used as primary antibodies and immunoreactive bands were captured and analyzed using a ChemiDoc (Bio-Rad) imaging system.

Techniques: Transgenic Assay, Inhibition, Control, Reverse Transcription Polymerase Chain Reaction